国产精华推荐2021,欧美性BBBBBXXXXXXX,国产乱妇无乱码大黄aa片,国产97在线 | 中文

當(dāng)前位置:首頁(yè)  >  技術(shù)文章  >  scRNA-seq和蛋白質(zhì)組學(xué)揭示了m2樣巨噬細(xì)胞在原發(fā)性和

scRNA-seq和蛋白質(zhì)組學(xué)揭示了m2樣巨噬細(xì)胞在原發(fā)性和

更新時(shí)間:2024-09-30  |  點(diǎn)擊率:594

20235月,福建醫(yī)科大學(xué)附屬醫(yī)院神經(jīng)外科;福建醫(yī)科大學(xué)附屬醫(yī)院福建省婦兒醫(yī)院婦科;莆田學(xué)院附屬醫(yī)院神經(jīng)外科;福州平潭綜合實(shí)驗(yàn)區(qū)醫(yī)院 (Department of Neurosurgery, Fujian Medical University Union Hospital,Fuzhou, China;Fujian Medical University, Fuzhou, China;Department of Gynaecology, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China;Department of Neurosurgery, Affiliated Hospital of Putian University, Putian,ChinaPingtan Comprehensive Experimental Area Hospital, Fuzhou, China) Guiting You老師研究團(tuán)隊(duì)在CNS Neuroscience & Therapeutics上發(fā)表論文:

scRNA-seq and proteomics reveal the distinction of M2-like macrophages between primary and recurrent malignant glioma and its critical role in the recurrence"


scRNA-seq和蛋白質(zhì)組學(xué)揭示了m2樣巨噬細(xì)胞在原發(fā)性和復(fù)發(fā)性惡性膠質(zhì)瘤中的差異及其在復(fù)發(fā)中的關(guān)鍵作用"


Abstract

Aims: Tumor-associated macrophages (TAMs) in the immune microenvironment play an important role in the increased drug resistance and recurrence of malignant glioma, but the mechanism remains incompletely inventoried. The focus of this study was to investigate the distinctions of M2-like TAMs in the immune microenvironment between primary and recurrent malignant glioma and its influence in the recurrence.

Methods: We employed single-cell RNA sequencing to construct a single-cell atlas for a total of 23,010 individual cells from 6 patients with primary or recurrent malignant glioma and identified 5 cell types, including TAMs and malignant cells. Immunohistochemical techniques and proteomics analysis were performed to investigate the role of intercellular interaction between malignant cells and TAMs in the recurrence of malignant glioma.

Results: Six subgroups of TAMs were annotated and M2-like TAMs were found to increase in recurrent malignant glioma significantly. A pseudotime trajectory and a dynamic gene expression profiling during the recurrence of malignant glioma were reconstructed. Up-regulation of several cancer pathways and intercellular interaction-related genes are associated with the recurrence of malignant glioma. Moreover, the M2-like TAMs can activate the PI3K/Akt/HIF-1α/CA9 pathway in the malignant glioma cells via SPP1-CD44-mediated intercellular interaction. Interestingly, high expression of CA9 can trigger the immunosuppressive response in the malignant glioma, thus promoting the degree of malignancy and drug resistance.

Conclusion: Our study uncovers the distinction of M2-like TAMs between primary and recurrent glioma, which offers unparalleled insights into the immune microenvironment of primary and recurrent malignant glioma.

摘要:

目的:免疫微環(huán)境中的腫瘤相關(guān)巨噬細(xì)胞(Tumor-associated macrophages, tam)在惡性膠質(zhì)瘤耐藥增加和復(fù)發(fā)中發(fā)揮重要作用,但其機(jī)制尚不全清楚。本研究的重點(diǎn)是探討原發(fā)性和復(fù)發(fā)性惡性膠質(zhì)瘤免疫微環(huán)境中m2樣tam的差異及其對(duì)復(fù)發(fā)的影響。

方法:采用單細(xì)胞RNA測(cè)序技術(shù),構(gòu)建來(lái)自6例原發(fā)性或復(fù)發(fā)性惡性膠質(zhì)瘤患者的23,010個(gè)細(xì)胞的單細(xì)胞圖譜,鑒定出包括tam細(xì)胞和惡性細(xì)胞在內(nèi)的5種細(xì)胞類型。通過(guò)免疫組織化學(xué)技術(shù)和蛋白質(zhì)組學(xué)分析,研究惡性細(xì)胞與tam之間的細(xì)胞間相互作用在惡性膠質(zhì)瘤復(fù)發(fā)中的作用。


結(jié)果:6個(gè)tam亞組被注釋,發(fā)現(xiàn)復(fù)發(fā)性惡性膠質(zhì)瘤中m2樣tam顯著增加。重建惡性膠質(zhì)瘤復(fù)發(fā)期間的假時(shí)間軌跡和動(dòng)態(tài)基因表達(dá)譜。幾種腫瘤通路和細(xì)胞間相互作用相關(guān)基因的上調(diào)與惡性膠質(zhì)瘤的復(fù)發(fā)有關(guān)。此外,m2樣tam可以通過(guò)spp1 - cd44介導(dǎo)的細(xì)胞間相互作用激活惡性膠質(zhì)瘤細(xì)胞中的PI3K/Akt/HIF-1α/CA9通路。有趣的是,在惡性膠質(zhì)瘤中,CA9的高表達(dá)可引發(fā)免疫抑制反應(yīng),從而促進(jìn)惡性程度和耐藥。

結(jié)論:研究人員的研究揭示了原發(fā)性和復(fù)發(fā)性膠質(zhì)瘤之間m2樣tam的差異,為原發(fā)性和復(fù)發(fā)性惡性膠質(zhì)瘤的免疫微環(huán)境提供了的見解。


該論文中,人膠質(zhì)瘤U87細(xì)胞及其慢病毒轉(zhuǎn)染細(xì)胞的體外培養(yǎng)是使用Ausbian特級(jí)胎牛血清完成的。



男男啪啪激烈高潮cc漫画免费| 日本丰满岳乱妇在线观看| 亚洲va中文字幕无码| 欧美丰满熟妇多毛XXXXX| 强奷漂亮少妇高潮A片| 无码专区人妻系列日韩精品| 色婷婷综合中文久久一本| 久久综合伊人77777麻豆| 中文无码一区二区三区在线观看| 无遮挡边摸边吃奶边做视频免费| 日本少妇被爽到高潮无码| 久久天天躁狠狠躁夜夜躁2014| 国产高清不卡一区二区| 日韩精品一区二区三区色欲AV| 亚洲av无码一区二区二三区软件| 熟女肥臀白浆大屁股一区二区| 亚洲成a人无码亚洲成a无码| 亚洲娇小与黑人巨大交| 丰满肥臀大屁股熟妇激情视频 | 久久久国产精品免费a片分环卫| 久久久久无码精品国产AV蜜桃| 99精品热这里只有精品| 少妇粉嫩小泬喷水视频www| 国产精品无码V在线观看| 久久亚洲中文字幕无码 | 少妇激情av一区二区| 欧美日韩国产精品自在自线| 国产偷窥熟女精品视频| 久久人人爽人人爽人人片av | 午夜性刺激免费看视频| 99麻豆久久久国产精品免费 | 亚洲av中文无码乱人伦在线r▽| 女人18毛片a级毛片免费视频 | 久久久久久亚洲精品不卡| 亚洲国产精品久久人人爱| 久久青青草原亚洲av无码麻豆| 国产精品久久久久久妇女| 亚洲AV无码片在线播放| 国产亚洲精久久久久久无码| 内射毛片内射国产夫妻| 国产熟女一区二区三区五月婷|